Plasmablasts, derived from the B-cell lineage and characterized as CD19lowCD20-CD38+CD27+, comprise a stage intermediate between activated B-cells and plasma cells. Plasmablasts are generally rare in the peripheral blood of healthy individuals, but expansions are observed briefly during responses to infection or vaccination Immunophenotypically, plasmablasts are cells which have lost their B cell phenotype, acquired the transcriptional and antigenic profile of PCs and usually also demonstrate a high proliferation rate [ 5, 16, 43, 45 ]. Combinations of B cell and PC markers are therefore employed to define plasmablastic differentiation within a B lineage lymphoma Plasmablastic lymphoma infiltrates in a diffuse pattern. The neoplastic cells are large and atypical with immunoblastic, plasmablastic, or plasmacytic features, including eccentric nuclei, prominent central or peripheral nucleoli, basophilic cytoplasm, and a perinuclear hof (Figure 8-15). Mature-appearing plasma cells may also be present
Supplemental Fig. 1. (a.) Usage of specific heavy chain V gene segments in patient repertoires for each cell population. CD19+ means total B cells, CD19+CD27+means memory B cells, and CD19+CD27 hi means plasmablasts. Black bars designate healthy donor samples, dark gray bars designate CIS-PTM memory B cells, light gray bars designate CIS-PTM plasmablasts, white bars designate NMO plasmablasts. Plasmablasts were co-infected by human herpesvirus 8 (HHV-8) and Epstein-Barr virus (EBV), being positive for HHV-8 (Fig. 1, inset left) and EBV by in situ hybridization (EBER) (Fig. 1, inset right). These cells were diffusely positive for CD38 (Fig. 2 , inset ), MUM1/IRF4, and immunoglobulin light chain lambda with a significant positivity for. The first two types of plasmacytoma, plasmacytic and plasmablastic, are fairly well defined from a cytologic perspective. The plasmacytic type (Fig. 1 A) consists mainly of mature or fairly mature plasma cells, usually arranged in solid sheets Plasmablastic lymphoma is an aggressive form of non-Hodgkin lymphoma. Although the condition most commonly occurs in the oral cavity, it can be diagnosed in many other parts of the body such as the gastrointestinal tract, lymph nodes, and skin
In the context of lymphoma pathogenesis and pathology, plasmablast is considered a deﬁning cell of PBL. In addition, prominent HHV8 þ immunoblast-like cells, also referred to as plasmablasts, were noted in multicentric plasma cell variant of Castleman disease (MCD) in HIV patients and HHV8 þ germi Plasmablastic myeloma is rare and morphologically similar to plasmablastic lymphoma, but is typically EBV-negative, and portends a poor prognosis with a reported median survival of 10 months.1 2 Given the low incidence of plasmablastic myeloma, there is no consensus on management of newly diagnosed patients. Treatment typically includes a combination of modern anti-myeloma agents, such as the. Ig light chains kappa and lambda are expressed by mature B cells and are an integral component of serum IgG with a ratio of approximately 2:1 kappa:lambda in humans. However, in IgA, the ratio is closer to 1:1 ( Koulieris et al., 2012). This skewed ratio of kappa to lambda light chains is also evident in memory B cells, plasmablasts, and. xenobiotics to induce a PBC-like pathology, including AMA, in animal models.(32-33) The enigma of PBC pathogenesis has been the specific targeting of the biliary epithelial cells in the setting of a ubiquitous autoantigen. The majority of AMA produced by plasmablasts is immunoglobulin A (IgA), which may undergo transcytosis through th
Crucial interactions occur between cells of the B-cell lineage (both B lymphocytes and plasmablasts) and two CD4+ T lymphocytes, the T-follicular helper (CD4+ Tfh) cells and CD4+ cytotoxic T lymphocytes (CD4+ CTL). This basic immunological understanding illuminates a number of potential targets for therapy Plasmablasts are a type of lymphoid cells that have retained the morphology of an immunoblast but have already acquired the immunophenotype of a plasma cell. Plasmablastic lymphoma may show plasma cells, but these are always reactive in nature and never neoplastic The plasmablasts are present in large numbers, involve germinal centers (germinotropism), are positive for MUM-1, LANA-1, and EBER, and are negative for CD20, CD79a, BCL-2, CD10 or BCL-6. Molecular analysis of the plasmablasts usually demonstrates a polyclonal or oligoclonal IgH gene rearrangement pattern . They divide rapidly and are still capable of internalizing antigens and presenting them to T cells.  A cell may stay in this state for several days, and then either die or irrevocably differentiate into a mature, fully differentiated plasma cell. [10 Background: Vaccinia is known to induce antibody and cellular responses. Plasmablast, circulating follicular helper T (cTFH) cells, cytokine-expressing CD4 T cells, and memory B cells were compared between subcutaneous (SC) and needle-free jet injection (JI) recipients of non-replicating modified vaccinia Ankara (MVA) vaccine. Methods: Vaccinia-naïve adults received MVA SC or by JI on.
CD38 antigen can deliver potent growth and differentiation signals to lymphoid and myeloid cells. It is found on immature cells of the B and T cell lineages but not on most mature resting peripheral lymphocytes. It is also present on thymocytes, pre-B cells, germinal center B cells, mitogen-activated T cells, Ig-secreting plasma cells. IgG4-related sclerosing disease is a syndrome characterized by the involvement of a wide variety of tissues by lymphoplasmacytic infiltrates and sclerosis, elevated serum IgG4 titer, and increased IgG4+ plasma cells in tissues. This report describes 2 cases with skin involvement, a feature not well Surgical Pathology Criteria Lambda-monoclonal plasmablasts HHV8+ B cell proliferation may progress to diffuse large B cell lymphoma, less commonly other lymphomas. May progress to plasmablastic lymphoma. Not associated with HIV. Highly associated with HIV Aims: To determine the utility of clinical, morphological and phenotypical features in the differential diagnosis of plasmablastic lymphoma and myeloma with plasmablastic features. Methods: All plasmablastic neoplasms identified from a 15-year retrospective search were reviewed and classified into 'lymphoma', 'myeloma' or 'indeterminate' Deshpande V, Zen Y, Chan JK, et al. Consensus statement on the pathology of IgG4-related disease. Mod Pathol 2012; 25:1181. Cheuk W, Chan JK. IgG4-related sclerosing disease: a critical appraisal of an evolving clinicopathologic entity. Adv Anat Pathol 2010; 17:303. Carruthers MN, Khosroshahi A, Augustin T, et al
Plasmablasts retain a proliferative capability together with an almost fully mature plasma cell phenotype . For a hematologic cytologist, a plasmablast is the circulating tumour cell observed in peripheral blood smears from a patient with plasma cell myeloma. None of the proposed markers on its own is able to sharpen the outlines of the. Plasmablasts are the lesional cells in both disorders and both may be HHV8 positive Stanford Medicine » School of Medicine » Departments » Surgical Pathology Criteria » KSHV-associated Germinotropic Lymphoproliferative Disorder . Surgical Pathology Criteria . Diagnostic Criteria. Pei Lin, L. Jeffrey Medeiros, in Gnepp's Diagnostic Surgical Pathology of the Head and Neck (Third Edition), 2021. Plasmablastic Lymphoma. In 1997, Delecluse and colleagues described a B-cell neoplasm with extensive plasmacytic differentiation, typically presenting in the oral cavity or nasopharynx of patients with HIV infection and AIDS. 133 They coined the term plasmablastic lymphoma (PBL. 1 Department of Pathology and Blood Bank, Prince Sultan Military Medical City, P.O. Box 7897, Riyadh 11159, Saudi Arabia. 2 Department of Hematology, Theodor Bilharz Research Institute, Egypt. 3 Hematopathology Division, Department of Basic Science, College of Medicine, Princess Nourah Bint Abdulrahman University, Riyadh, Saudi Arabia. 4 Department of Oncology, Prince Sultan Military Medical. Pathology Findings Morphologic features and diagnoses. The 17 cases of plasmablastic neoplasm showed a variety of morphologic features with at least a subset of plasmablasts present in all cases
(A) Experimental outline. (B) Sorting strategy. IgM - plasmablasts were identified as CD19 + CD27 ++ CD38 ++ and either IgG + or IgA +. (C) Frequency of total plasmablasts of each isotype as detected during cell sorting and through anti-Ig ELISPOT. The ELISPOT data also report the frequency of vaccine-specific plasmablasts (QIV) (a) Outline of the 2014/15 season study where RNA was purified from memory B cells and total PBMCs at days 0, 28 and 90 after vaccination, and from HA-positive and total ABCs and total ASCs at day. Department of Oral Pathology and Microbiology, Dr. D. Y. Patil Dental College and Hospital, Pimpri, Pune 411018, India monomorphic population of plasmablasts with no or mini- nuclear outlines . Plasmablasts are a type of lymphoi
Plasmablastic lymphoma (PBL) is a distinctive B-cell neoplasm that shows diffuse proliferation of large neoplastic cells, most of which resemble B-immunoblasts and have immunophenotype of plasma cells. 1 PBL was originally described as a rare variant of diffuse large B cell lymphoma (DLBCL) involving the oral cavity and occurring in the clinical setting of HIV and latent EBV infection. 2,3 PBL. Oral plasmablastic lymphoma (PBL) is a rare malignancy, associated with HIV or other immunocompromised conditions. The lesion constituted a new subtype of diffuse large B-cell lymphoma and proposed a distinct entity based on its basic morphology, its clinical behaviour involving predominantly extramedullary sites (particularly oral cavity), and its limited antigenic phenotype data suggesting. Longitudinal analysis reveals that delayed bystander CD8+ T cell activation and early immune pathology distinguish severe COVID-19 from mild disease. but increased circulating plasmablasts (Arunachalam et al., 2020; We will outline the major datasets collected in this study before integrating them to study early and recovering disease 1 Department of Pathology and Laboratory Medicine, Figure 1 outlines possible pathways of MALT lymphomagenesis. (plasmablasts) as well as pleomorphism with bi-nucleated and frequently trinucleated forms is typically noted in advanced or more extensive disease process. The neoplastic plasma cells express plasma cell markers such as CD138.
Kawasaki disease is a systemic vasculitis that affects infants and young children 1,2,3.Kawasaki disease is now the leading cause of acquired heart disease among children in North America, Europe. The lymph nodes are organized lymphoid organs that contain lymphocytes within a fine reticular stroma. The structures within a lymph node include the capsule, subcapsular sinus, cortex (B cell zone with follicles and germinal centers), paracortex (T cell zone), medullary sinuses, medullary cords and hilus
1 DISEASE OVERVIEW. Epstein Barr virus-positive (EBV+) diffuse large B-cell lymphoma (DLBCL), not otherwise specified (NOS), is a clinicopathological entity recognized in the revised 4th edition of the 2016 classification of the World Health Organization (WHO). 1 The seminal report by Oyama and colleagues reported on 22 patients with large cell lymphoma that expressed the EBV-encoded RNA (EBER. February 2014 - Volume 46 - Abstracts: RCPA Pathology Update 2014 IAP Australasian Division 38th Annual Scientific Meeting pp: S3-S120 Table of Contents Section
emerging new CD4 + T-cell subsets. Purpose of review New insights into IgG4-related disease (IgG4-RD) have recently been obtained. A better understanding of the mechanisms underlying this disease is important for identification of therapeutic targets, which will lead to the development of specific strategies for treatment . The disease can affect almost any anatomic location, but the sites involved most commonly are the pancreas, salivary glands, orbital adnexa, lymph nodes, and retroperitoneum. IgG4-RD, typically diagnosed among individuals who are.
Intravascular large B-cell lymphoma (IVLBCL), whose B-cell origin was demonstrated ∼30 years ago, 1 is a rare lymphoma entity characterized by the predominant, if not exclusive, growth of large cells within the lumen of different-sized blood vessels. 2 Behind this relatively intuitive definition, however, many unclear aspects persist. Even though the fundamental peculiarity of the. Systemic lupus erythematosus (SLE) is a chronic inflammatory disease with various clinical features. Autoreactive B cells play a role in disease pathogenesis, through the production of multiple autoantibodies, which form immune complexes and induce the inflammatory response and tissue damage associated with SLE. Recently, tetraspanins, and in particular, TSPAN32, have been recognized to play a. B cells, also known as B lymphocytes, are a type of white blood cell of the lymphocyte subtype. They function in the humoral immunity component of the adaptive immune system. B cells produce antibody molecules; however, these antibodies are not secreted. Rather, they are inserted into the plasma membrane where they serve as a part of B-cell receptors. When a naïve or memory B cell is. Although BLIMP1h plasma cells derive from BLIMP1mid cells, it remains unknown whether plasma cells are derived from plasmablasts (indicated by the dashed arrow) or from an earlier plasma cell-committed stage. The contribution of marginal zone B cells and B1 cells to the long-lived plasma cell compartment is also poorly characterized . We present, to the best of our knowledge, the first extensive review of the epidemiology, clinical findings, pathology, and outcome of HIV-associated PBL. Methods: We identified 131 cases of PBL in HIV- positive.
Pathology of NMOSD lesions is driven by the binding of pathogenic AQP4 Ab on astrocytic 6 receptor has demonstrated a strong potential to block this pathway through reducing the survival of AQP4 Ab-secreting plasmablasts Evidence of disease initiation and exacerbation following passive transfer of AQP4 Ab further outlines the pathogenic. Outline Infectious mononucleosis Kikuchi-Fujimoto lymphadenitis Castleman disease Intravascular lymphocytosis Seminoma Nasopharynealcarcinoma Thymoma Myeloid sarcoma Infectious Mononucleosis Basic Facts Caused by Epstein-Barr virus (HHV-4) Spread by contact with human secretions (Saliva to oral epithelium to B-cells) Age of contact depends on. Plasma cell dyscrasias (also termed plasma cell disorders and plasma cell proliferative diseases) are a spectrum of progressively more severe monoclonal gammopathies in which a clone or multiple clones of pre-malignant or malignant plasma cells (sometimes in association with lymphoplasmacytoid cells or B lymphocytes) over-produce and secrete into the blood stream a myeloma protein, i.e. an. Although HIV-related infectious and neoplastic GI pathology is characteristically seen in immunocompromised patients, drug-associated disorders may occur at any stage of HIV disease and involve any level of the GI tract. Table 2 outlines the most important GI histopathology/injury patterns that may be seen in this patient population
Results A PD-1 + CXCR5 − CD4 + Th-CXCL13 cell subset was identified in NPC. This subset was a major source of CXCL13, representing the majority of the CD4 + T cells at levels comparable with Th1 and Tfh cells present in the TLSs. Monocytes activated by toll-like receptor 4 agonists served as the antigen-presenting cells that most efficiently triggered the expansion of Th-CXCL13 cells MARCO (Invitrogen, PA5-26888, 1:300) and CD68 (DAKO, PG-M1, 1:600) staining was performed on sequentially cut slides by the Toronto Pathology Research Program (Toronto General Hospital) using. By contrast, disruption of ICOS-ICOSL signalling in mice prevents autoantibody formation and end-organ pathology in mice with collagen-induced arthritis 115,116,117, myasthenia gravis 118 and. Outline strategies for early and accurate diagnosis of neuromyelitis opticaspectrum disorder (NMOSD) Describe how novel treatment options target the pathophysiology of NMOSD to prevent relapse IL-6, plasmablasts and NMOSD pathology1. It did not appear that contamination from circulating plasmablasts influenced this result. These cells persisted in 5 patients who underwent a second bone marrow sample at 11 months after infection, and IgG titers among these patients also remained stable between 7 and 11 months postinfection
Although the generation of long-lived PCs in response to foreign antigens was conclusively shown some time ago (4, 5), the first clear demonstration that such cells are produced during an autoimmune response is provided in the article of Hoyer et al. in this issue (pages 1577-1584) ().They show in a mouse model of SLE that a large fraction of the PC-producing anti-self-antibodies (anti-DNA. International consensus guidelines outline the histopathologic and immunohistochemistry features that support the diagnosis of IgG4-RD and, in the proper clinical setting, can be viewed as diagnostic 26, 27. Needle biopsy is usually inadequate for the histopathologic diagnosis of IgG4-RD, but generally yields quantities of tissue large enough. Synovial Cavity. The synovial cavity is normally only a potential space with 1-2ml of highly viscous (due to hyaluronic acid) fluid with few cells. In RA, large collections of fluid (effusions) occur which are, in effect, filtrates of plasma (and, therefore, exudative - i.e., high protein content). The synovial fluid is highly. 3) Lymphocyte-rich- reactive lymphocytes make up the majority of the cellular infiltrate, frequent RS cells, assc. w/ EBV in 40%. 4) Lymphocyte depletion- least common type, paucity of lymphocytes & RS cells, RS cells are infected w/ EBV in > 90%, occurs in older individuals, less favorable prognosis
The red pulp is composed of a three dimensional meshwork of splenic cords and venous sinuses. The splenic cords are composed of reticular fibers, reticular cells, and associated macrophages (Saito et al., 1988).The reticular cells are considered to be myofibroblasts and may play a role in splenic contraction (Saito et al., 1988).With electron microscopy, it is apparent that the reticular. The diagnosis of multiple myeloma is based on a combination of radiological, laboratory and pathological findings. Bone marrow plasmocytosis is the most important criterion in the diagnosis of multiple myeloma. The pattern of infiltration and the plasma cell morphology have prognostic significance. The immunophenotype of the plasma cells can change after treatment; a polytypic phenotype after. Lymph node biopsy is usually undertaken to investigate possible neoplastic involvement and it is appropriate that most emphasis in the teaching of lymph node pathology is placed on recognizing neoplastic diseases correctly. However, it is important also to recognize the suggestive and sometimes specific changes that occur in lymph nodes due to infection
In tissue samples, HHV8‐infected plasmablasts localise to the mantle zones of the lymphoid follicles. We revisited the immunohistological features in 25 lymph node (LN) and three spleen samples of MCD. In five (20%) LN and one (33%) spleen sample, HHV8 latent nuclear antigen 1 (LANA1) staining was also noted on the follicular dendritic cells. These plasmablasts are lambda-light chain restricted, uniformly express cytoplasmic IgM, and may colonize the germinal centers (Figures 1E and 1F). 18,45-47 They are polyclonal and have heterogeneous, weak expression of CD20. 45,48-51 Localized collections of HHV8-positive plasmablasts may occur, referred to by some as microlymphomas. 52. Free Online Library: The Pathology of Reactive Lymphadenopathies: A Discussion of Common Reactive Patterns and Their Malignant Mimics.(Special Section-Contributions From the Canadian Anatomic and Molecular Pathology Conference (CAMP), Part II) by Archives of Pathology & Laboratory Medicine; Health, general B cells Non-Hodgkin's lymphomas Diagnosi Plasmablasts are lymphoid cells that morphologically resemble B-cell immunoblasts but have acquired a plasma cell immunophenotype (i.e., loss of B-cell markers and surface immunoglobulin with the acquisition of plasma cell surface markers). A 35-year-old, apparently healthy individual reported to the Department of Oral Pathology complaining.
Findings. We report that the alterations in B cell subsets observed in acute COVID-19 patients were largely recovered in convalescent patients. In contrast, T cells from convalescent patients displayed continued alterations with persistence of a cytotoxic program evident in CD8 + T cells as well as elevated production of type 1 cytokines and interleukin-17 (IL-17) Metastases to the thyroid are rare, despite the rich vascular supply of the gland. Metastases account for approximately 7.5% of thyroid malignancies. 1 The main sites of origin are the lungs, esophagus, breasts, and kidneys. 2,3 A monoclonal proliferation of plasma cell origin can be isolated, as seen in extraosseous plasmacytoma or in the context of multiple myeloma. 4 The extension of a.
The immune changes that underlie COVID-19 severity have not been fully defined. By analyzing a longitudinal cohort of COVID-19 patients and integrating inflammatory factors, immunophenotyping, and transcriptome data, Bergamaschi et al. identify both early and persistent immune changes that distinguish mild and/or asymptomatic from more severe disease Subsequently, these activated B cells may differentiate into antibody secreting cells in circulation (plasmablasts), where importantly they downregulate CD20, the target of rituximab. 89 The plasmablasts which reach bone marrow niches have also often downregulated CD19 and expressed CD138. 90 One important question is the degree to which these. This Review outlines a practical approach to assessing and managing polyclonal hypergammaglobulinaemia in adults. Polyclonal hypergammaglobulinaemia is most commonly caused by liver disease, immune dysregulation, or inflammation, but can also provide an important diagnostic clue of rare diseases such as histiocyte disorders, autoimmune lymphoproliferative syndrome, Castleman disease, and IgG4.
long-lived, antibody-producing plasma cells. In transplantation, plasma cells produce antibodies directed against human leukocyte antigen (HLA) antigens causing poor allograft survival. We report the first clinical experience with a plasma cell depleting therapy, bortezomib, to abrogate anti-HLA antibodies in transplantation (outside of rejection) in an attempt to improve long-term allograft. Myasthenia gravis (MG) is the prototypical autoimmune disorder caused by specific autoantibodies at the neuromuscular junction. Broad-based immunotherapies, such as corticosteroids, azathioprine, mycophenolate, tacrolimus, and cyclosporine, have been effective in controlling symptoms of myasthenia. While being effective in a majority of MG patients many of these immunosuppressive agents are. Purpose Biologic heterogeneity is a feature of diffuse large B-cell lymphoma (DLBCL), and the existence of a subgroup with poor prognosis and phenotypic proximity to Burkitt lymphoma is well known. Conventional cytogenetics identifies some patients with rearrangements of MYC and BCL2 and/or BCL6 (double-hit lymphomas) who are increasingly treated with more intensive chemotherapy, but a more. Castleman disease (CD) is a rare disease of lymph nodes and related tissues. It is a heterogenous group of lymphoproliferative disorders that are sometimes associated with human immunodeficiency virus (HIV) and human herpesvirus 8 (HHV-8)
Departments of Pathology, dagger Oral and Maxillofacial Pathology, and double dagger Internal Medicine, Ohio State University Medical Center and College of Dentistry, Columbus, OH. Am J Surg Pathol. 2004 Jan; 28(1): 41-6 We have created a genomic pathology curriculum as a first step in helping pathology residents build a foundation for the understanding of genomic medicine and its implications for clinical practice. This curriculum is freely accessible online. View details for DOI 10.1016/j.jmoldx.2012.11.003. View details for Web of Science ID 00031584160000 Medium-sized lymphocytes with clear cytoplasm, indented nuclei, and large lymphoid cells scattered at the periphery of the nodules. CD20 and CD19 positive, CD43 positive or negative, positive for CD11c, negative for CD103 and CD123; variable positivity for CD25 and CD200. Splenic diffuse red pulp small B-cell lymphoma Department of Pathology, Seoul National University College of Medicine, Korea. J Korean Med Sci 2000 Aug;15(4):393-8 Abstract quote Castleman's disease represents an atypical lymphoproliferative disorder, infrequently associated with various immunologic abnormalities or subsequent development of malignancy such as Kaposi sarcoma, malignant. Diagnostic Lymph Node Pathology This page intentionally left blank Diagnostic Lymph Node Pathology Dennis H. Wright 788 320 26MB Pages 171 Page size 525 x 635 pts Year 201
Departments of Pathology (MB, NJA, AS, ET, SK) and Anatomy-Histology-Embryology (VG, PK), Medical Faculty, University of Ioannina, Ioannina, Greece. Mod Pathol 2003 May;16(5):471-80 Abstract quote There is increasing evidence that bcl6 and CD10 expression may be related to apoptosis and cell cycle progression Plasmablasts were deﬁned as being negative for the lineage marker (CD3, CD14, and CD16), the dendritic cell markers CD11c and CD123, and sIgG but positive for HLA-DR (HLA-DR⫹), Ki-67 (Ki-67⫹), and ICIgG (ICIgG⫹). Plasmablasts exhibited no or only intermediate expression of CD20 (CD20⫺/int) Most patients with primary immune deficiency suffer from recurrent infections; however, paradoxical autoimmune phenomena can also manifest. The aim of this study was to identify immunological markers of autoimmune phenomena associated with common variable immunodeficiency (CVID). The study included 33 adults with CVID divided into two groups: (1) those with noninfectious autoimmune.